Hydrodynamic Description of Granular Convection

We present a hydrodynamic model that captures the essence of granular dynamics in a vibrating bed. We carry out the linear stability analysis and uncover the instability mechanism that leads to the appearance of the convective rolls via a supercritical bifurcation of a bouncing solution. We also explicitly determine the onset of convection as a function of control parameters and confirm our picture by numerical simulations of the continuum equations.Comment: 14 pages, RevTex 11pages + 3 pages figures (Type csh

Immunoregulatory functions for murine intraepithelial lymphocytes: gamma/delta T cell receptor-positive (TCR+) T cells abrogate oral tolerance, while alpha/beta TCR+ T cells provide B cell help.

Past work has shown that a subset of effector T cells with unique characteristics could abrogate hapten- or antigen-induced tolerance, and the reconstitution of this immune response has been termed contrasuppression. We have studied contrasuppression in a model of oral tolerance (OT) in which adoptively transferred antigen-specific T contrasuppressor (Tcs) cells reverse OT and result in antibody responses to the eliciting antigen. In the present study, we show that murine intraepithelial lymphocytes (IELs) from mice orally immunized with sheep red blood cells (SRBC) contain T cells that exhibit Tcs cell activity. This effect was mediated by CD3+ gamma/delta T cell receptor-positive (TCR+), but not alpha/beta TCR+ T cells, and gamma/delta TCR+ Tcs cells were associated with both the CD4-,CD8+ and CD4-,CD8- (double-negative) IEL fractions. The CD4-,CD8+ gamma/delta TCR+ IELs were further separated into Vicia villosa-adherent and -nonadherent fractions. Adoptive transfer of V. villosa-adherent gamma/delta TCR+ T cells to mice with OT to SRBC resulted in splenic IgA, IgM, and IgG subclass anti-SRBC responses, while V. villosa-nonadherent gamma/delta TCR+ T cells were without activity. The gamma/delta TCR+ IELs did not support in vitro antibody responses in B cell cultures, while alpha/beta TCR+ IELs were effective T helper cells. Further, cytokine production by the gamma/delta TCR+ IELs was examined, and the gamma/delta TCR+ V. villosa-adherent fraction, which possessed contrasuppressor function, contained low levels of IL-5 mRNA and small numbers of IL-5-producing cells when compared with alpha/beta TCR+ IELs and V. villosa-nonadherent gamma/delta TCR+ IELs. Our results now show that mouse IELs contain two distinct types of T cells that function in the immune response, e.g., alpha/beta TCR+ T cells that produce IL-5 and function as helper cells, and gamma/delta TCR+ T cells that restore antibody responses in mice that had been orally tolerized with antigen

Ozone-enhanced layers in the troposphere over the equatorial Pacific Ocean and the influence of transport of midlatitude UT/LS air

International audienceOccurrence of ozone (O3)-enhanced layers in the troposphere over the equatorial Pacific Ocean and their seasonal variation were investigated based on ozonesonde data obtained at three Southern Hemisphere ADditional OZonesondes (SHADOZ) sites, Watukosek, American Samoa and San Cristobal, for 6 years between 1998 and 2003. O3-enhanced layers were found in about 50% of observed O3 profiles at the three sites on yearly average. The formation processes of O3-enhanced layers were investigated by meteorological analyses including backward trajectories. On numerous occasions, O3-enhanced layers resulted from the transport of air masses affected by biomass burning. The contribution of this process was about 30% at San Cristobal during the periods from February to March and from August to September, while it was relatively low, about 10%, at Watukosek and Samoa. A significant number of the O3-enhanced layers were attributed to the transport of midlatitude upper-troposphere and lower-stratosphere (UT/LS) air. Meteorological analyses indicated that these layers originated from equatorward and downward transport of the midlatitude UT/LS air masses through a narrow region between high- and low-pressure systems around the subtropical jet stream. This process accounted for more than 40% at Watukosek between May and December, about 60% or more at Samoa all year around, and about 40% at San Cristobal between November and March, indicating that it was important for O3 budget over the equatorial Pacific Ocean

Localization of Alkaline Phosphatase and Cathepsin D during Cell Restoration after Colchicine Treatment in Primary Cultures of Fetal Rat Hepatocytes

Localization of alkaline phosphatase (ALP) and cathepsin D (CAPD) in primary cultures of fetal rat hepatocytes was examined using double immunofluorescent staining in order to investigate the relationship between lysosome movement and the fate of ALP during cell restoration after microtubule disruption by colchicine. At 3 hr and 24 hr after colchicine treatment, numerous coarse dots containing ALP were observed throughout the cytoplasm, and some of these showed colocalization with CAPD. At 48 hr and 72 hr after colchicine treatment, although most of the dots containing ALP in the cytoplasm disappeared, dots containing CAPD remained. The present results suggest that the denatured ALP proteins remaining in the cytoplasm of hepatocytes during cell restoration after colchicine treatment are digested by lysosomes

Determination of the Joint Confidence Region of Optimal Operating Conditions in Robust Design by Bootstrap Technique

Robust design has been widely recognized as a leading method in reducing variability and improving quality. Most of the engineering statistics literature mainly focuses on finding "point estimates" of the optimum operating conditions for robust design. Various procedures for calculating point estimates of the optimum operating conditions are considered. Although this point estimation procedure is important for continuous quality improvement, the immediate question is "how accurate are these optimum operating conditions?" The answer for this is to consider interval estimation for a single variable or joint confidence regions for multiple variables. In this paper, with the help of the bootstrap technique, we develop procedures for obtaining joint "confidence regions" for the optimum operating conditions. Two different procedures using Bonferroni and multivariate normal approximation are introduced. The proposed methods are illustrated and substantiated using a numerical example.Comment: Two tables, Three figure